4N8C

Three-dimensional structure of the extracellular domain of Matrix protein 2 of influenza A virus

Summary for 4N8C

• Entry DOI: 10.2210/pdb4n8c/pdb
• Descriptor: Heavy chain of monoclonal antibody, Light chain of monoclonal antibody, Extracellular domain of influenza Matrix protein 2, ... (4 entities in total)
• Functional Keywords: antibody-peptide complex, influenza a virus protein, immune system
• Biological source: Mus musculus; More
• Total number of polymer chains: 6
• Total formula weight: 101817.37

Authors

Cho, K.J.,Seok, J.H.,Kim, S.,Roose, K.,Schepens, B.,Fiers, W.,Saelens, X.,Kim, K.H. (deposition date: 2013-10-17, release date: 2014-10-22, Last modification date: 2024-10-30)

Primary citation

Cho, K.J.,Schepens, B.,Seok, J.H.,Kim, S.,Roose, K.,Lee, J.H.,Gallardo, R.,Hamme, E.V.,Schymkowitz, J.,Rousseau, F.,Fiers, W.,Saelens, X.,Kim, K.H.
Structure of the extracellular domain of matrix protein 2 of influenza A virus in complex with a protective monoclonal antibody
J.Virol., 89:3700-3711, 2015

PubMed Abstract: The extracellular domain of influenza A virus matrix protein 2 (M2e) is conserved and is being evaluated as a quasiuniversal influenza A vaccine candidate. We describe the crystal structure at 1.6 Å resolution of M2e in complex with the Fab fragment of an M2e-specific monoclonal antibody that protects against influenza A virus challenge. This antibody binds M2 expressed on the surfaces of cells infected with influenza A virus. Five out of six complementary determining regions interact with M2e, and three highly conserved M2e residues are critical for this interaction. In this complex, M2e adopts a compact U-shaped conformation stabilized in the center by the highly conserved tryptophan residue in M2e. This is the first description of the three-dimensional structure of M2e.

PubMed: 25609808
DOI: 10.1128/JVI.02576-14

Experimental method

X-RAY DIFFRACTION (1.6 Å)