4N8C
Three-dimensional structure of the extracellular domain of Matrix protein 2 of influenza A virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.353, 72.276, 78.446 |
| Unit cell angles | 86.88, 77.53, 84.63 |
Refinement procedure
| Resolution | 38.430 - 1.600 |
| R-factor | 0.1957 |
| Rwork | 0.193 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbm |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.955 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.054 | 0.035 | 0.674 |
| Number of reflections | 109543 | ||
| <I/σ(I)> | 13.9 | ||
| Completeness [%] | 96.9 | 97.9 | 95.2 |
| Redundancy | 3.9 | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 20% PEG3350, 200 mM Sodium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
