Crystal structure of Hyp-1, a St John's wort PR-10 protein, in complex with 8-anilino-1-naphthalene sulfonate (ANS)

Summary for 4N3E

Related3IE5 1ICX 1IFV 1XDF 2FLH 2QIM 3E85 4GY9 4JHG 3US7 1BV1 4A80 4A81 4A86 4A85 4A83 4A8V 1FM4 4A8U 4A87
DescriptorPhenolic oxidative coupling protein, 8-ANILINO-1-NAPHTHALENE SULFONATE, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (5 entities in total)
Functional Keywordsplant hormone binding, phytohormone binding, cytokinin, plant defense, pathogenesis-related protein, pr-10 protein, hypericin, depression, pr-10 fold, hydrophobic cavity, glycine-rich loop, ans displacement assay (ada), commensurately modulated superstructure, tetartohedral twinning, plant protein
Biological sourceHypericum perforatum (St. John's wort)
Total number of polymer chains28
Total molecular weight545269.47
Sliwiak, J.,Dauter, Z.,Mccoy, A.J.,Read, R.J.,Jaskolski, M. (deposition date: 2013-10-07, release date: 2014-02-26, Last modification date: 2017-11-15)
Primary citation
Sliwiak, J.,Jaskolski, M.,Dauter, Z.,McCoy, A.J.,Read, R.J.
Likelihood-based molecular-replacement solution for a highly pathological crystal with tetartohedral twinning and sevenfold translational noncrystallographic symmetry.
Acta Crystallogr.,Sect.D, 70:471-480, 2014
PubMed: 24531481 (PDB entries with the same primary citation)
DOI: 10.1107/S1399004713030319
MImport into Mendeley
Experimental method

Structure validation

RfreeRamachandran outliersSidechain outliersRSRZ outliers 0.284 1.2% 9.5% 2.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-10-14