4N31

Structure and activity of Streptococcus pyogenes SipA: a signal peptidase homologue essential for pilus polymerisation

Summary for 4N31

• Entry DOI: 10.2210/pdb4n31/pdb
• Related: 4K8W
• Descriptor: SipA, PHOSPHATE ION, PHOSPHATIDYLETHANOLAMINE, ... (4 entities in total)
• Functional Keywords: streptococcus pyogenes, pilus polymerisation, signal peptidase family, pilin assembly protein, bacterial cell membrane, extracellular, cell adhesion
• Biological source: Streptococcus pyogenes
• Total number of polymer chains: 2
• Total formula weight: 38197.64

Authors

Young, P.G.,Proft, T.,Baker, E.N. (deposition date: 2013-10-06, release date: 2014-08-20, Last modification date: 2023-09-20)

Primary citation

Young, P.G.,Proft, T.,Harris, P.W.,Brimble, M.A.,Baker, E.N.
Structure and activity of Streptococcus pyogenes SipA: a signal peptidase-like protein essential for pilus polymerisation.
Plos One, 9:e99135-e99135, 2014

PubMed Abstract: The pili expressed on the surface of the human pathogen Streptococcus pyogenes play an important role in host cell attachment, colonisation and pathogenesis. These pili are built from two or three components, an adhesin subunit at the tip, a major pilin that forms a polymeric shaft, and a basal pilin that is attached to the cell wall. Assembly is carried out by specific sortase (cysteine transpeptidase) enzyme. These components are encoded in a small gene cluster within the S. pyogenes genome, often together with another protein, SipA, whose function is unknown. We show through functional assays, carried out by expressing the S. pyogenes pilus components in Lactococcus lactis, SipA from the clinically important M1T1 strain is essential for pilus assembly, and that SipA function is likely to be conserved in all S. pyogenes. From the crystal structure of SipA we confirm that SipA belongs to the family of bacterial signal peptidases (SPases), which process the signal-peptides of secreted proteins. In contrast to a previous arm-swapped SipA dimer, this present structure shows that its principal domain closely resembles the catalytic domain of SPases and has a very similar peptide-binding cleft, but it lacks the catalytic Ser and Lys residues characteristic of SPases. In SipA these are replaced by Asp and Gly residues, which play no part in activity. We propose that SipA functions by binding a key component at the bacterial cell surface, in a conformation that facilitates pilus assembly.

PubMed: 24911348
DOI: 10.1371/journal.pone.0099135

Experimental method

X-RAY DIFFRACTION (2.2 Å)