Crystal structure of Thermus thermophilus RNA polymerase holoenzyme in complex with GE23077

Summary for 4MQ9

Related PRD IDPRD_001181
DescriptorDNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (10 entities in total)
Functional Keywordsdna-directed rna polymerase, transcription, transferase-antibiotic complex, transferase/antibiotic
Biological sourceThermus thermophilus
Cellular locationCytoplasm (By similarity) Q5SKW1
Total number of polymer chains7
Total molecular weight429585.67
Ho, M.X.,Arnold, E.,Ebright, R.H.,Zhang, Y.,Tuske, S. (deposition date: 2013-09-16, release date: 2014-05-07, Last modification date: 2014-05-28)
Primary citation
Zhang, Y.,Degen, D.,Ho, M.X.,Sineva, E.,Ebright, K.Y.,Ebright, Y.W.,Mekler, V.,Vahedian-Movahed, H.,Feng, Y.,Yin, R.,Tuske, S.,Irschik, H.,Jansen, R.,Maffioli, S.,Donadio, S.,Arnold, E.,Ebright, R.H.
GE23077 binds to the RNA polymerase 'i' and 'i+1' sites and prevents the binding of initiating nucleotides.
Elife, 3:e02450-e02450, 2014
PubMed: 24755292 (PDB entries with the same primary citation)
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.24180.0%3.7%1.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4mq9
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Molmil generated image of 4mq9
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Molmil generated image of 4mq9
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