4MQ9
Crystal structure of Thermus thermophilus RNA polymerase holoenzyme in complex with GE23077
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.91800 |
Spacegroup name | P 65 |
Unit cell lengths | 236.569, 236.569, 252.311 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.971 - 3.350 |
R-factor | 0.2143 |
Rwork | 0.214 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dxj |
RMSD bond length | 0.004 |
RMSD bond angle | 0.846 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.760 | 3.530 |
High resolution limit [Å] | 3.350 | 3.350 |
Rmerge | 0.112 | 1.000 |
Number of reflections | 113506 | |
<I/σ(I)> | 8.5428 | 0.76 |
Completeness [%] | 99.1 | 97.2 |
Redundancy | 5.81 | 5.39 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | A stock crystallization solution was prepared by adding T. thermophilus RNAP holoenzyme (10 mg/ml) in 20 mm TRIS-HCl, ph 7.7, 100 mm NaCl, and 1% glycerol to an equal volume of 33 mm magnesium formate containing 40 m ZnCl2. This solution was equilibrated against 35 mm magnesium formate and 30 mm sodium citrate, ph 5.4, vapor diffusion, hanging drop, temperature 295K, VAPOR DIFFUSION, HANGING DROP |