4M8O

TERNARY COMPLEX OF DNA POLYMERASE EPSILON WITH AN INCOMING dATP

4M8O の概要

• エントリーDOI: 10.2210/pdb4m8o/pdb
• 分子名称: DNA polymerase epsilon catalytic subunit A, PRIMER DNA, TEMPLATE DNA, ... (9 entities in total)
• 機能のキーワード: dna polymerase b type, dna synthesis, protein-dna complex, dna replication, metal-binding, transferase dna complex, nucleotidyltransferase, transferase-dna complex, transferase/dna
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleus: P21951
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 152226.41

構造登録者

Sauer-Eriksson, A.E.,Hogg, M.,Osterman, P.,Johansson, E. (登録日: 2013-08-13, 公開日: 2013-11-27, 最終更新日: 2023-09-20)

主引用文献

Hogg, M.,Osterman, P.,Bylund, G.O.,Ganai, R.A.,Lundstrom, E.B.,Sauer-Eriksson, A.E.,Johansson, E.
Structural basis for processive DNA synthesis by yeast DNA polymerase epsilon.
Nat.Struct.Mol.Biol., 21:49-55, 2014

PubMed Abstract: DNA polymerase ɛ (Pol ɛ) is a high-fidelity polymerase that has been shown to participate in leading-strand synthesis during DNA replication in eukaryotic cells. We present here a ternary structure of the catalytic core of Pol ɛ (142 kDa) from Saccharomyces cerevisiae in complex with DNA and an incoming nucleotide. This structure provides information about the selection of the correct nucleotide and the positions of amino acids that might be critical for proofreading activity. Pol ɛ has the highest fidelity among B-family polymerases despite the absence of an extended β-hairpin loop that is required for high-fidelity replication by other B-family polymerases. Moreover, the catalytic core has a new domain that allows Pol ɛ to encircle the nascent double-stranded DNA. Altogether, the structure provides an explanation for the high processivity and high fidelity of leading-strand DNA synthesis in eukaryotes.

PubMed: 24292646
DOI: 10.1038/nsmb.2712

実験手法

X-RAY DIFFRACTION (2.2 Å)