4M7D

Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU

4M7D の概要

• エントリーDOI: 10.2210/pdb4m7d/pdb
• 関連するPDBエントリー: 4M75 4M77 4M78 4M7A
• 分子名称: U6 snRNA-associated Sm-like protein LSm8, U6 snRNA-associated Sm-like protein LSm2, U6 snRNA-associated Sm-like protein LSm3, ... (9 entities in total)
• 機能のキーワード: sm like fold, rna splicing, structural protein-rna complex, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleus: P47093 P38203 P57743 Q06406 P40089 P40070; Cytoplasm: P53905
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 155654.79

構造登録者

Zhou, L.,Hang, J.,Zhou, Y.,Wan, R.,Lu, G.,Yan, C.,Shi, Y. (登録日: 2013-08-12, 公開日: 2013-11-13, 最終更新日: 2023-09-20)

主引用文献

Zhou, L.,Hang, J.,Zhou, Y.,Wan, R.,Lu, G.,Yin, P.,Yan, C.,Shi, Y.
Crystal structures of the Lsm complex bound to the 3' end sequence of U6 small nuclear RNA.
Nature, 506:116-120, 2014

PubMed Abstract: Splicing of precursor messenger RNA (pre-mRNA) in eukaryotic cells is carried out by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs) and a number of accessory factors and enzymes. Each snRNP contains a ring-shaped subcomplex of seven proteins and a specific RNA molecule. The U6 snRNP contains a unique heptameric Lsm protein complex, which specifically recognizes the U6 small nuclear RNA at its 3' end. Here we report the crystal structures of the heptameric Lsm complex, both by itself and in complex with a 3' fragment of U6 snRNA, at 2.8 Å resolution. Each of the seven Lsm proteins interacts with two neighbouring Lsm components to form a doughnut-shaped assembly, with the order Lsm3-2-8-4-7-5-6. The four uridine nucleotides at the 3' end of U6 snRNA are modularly recognized by Lsm3, Lsm2, Lsm8 and Lsm4, with the uracil base specificity conferred by a highly conserved asparagine residue. The uracil base at the extreme 3' end is sandwiched by His 36 and Arg 69 from Lsm3, through π-π and cation-π interactions, respectively. The distinctive end-recognition of U6 snRNA by the Lsm complex contrasts with RNA binding by the Sm complex in the other snRNPs. The structural features and associated biochemical analyses deepen mechanistic understanding of the U6 snRNP function in pre-mRNA splicing.

PubMed: 24240276
DOI: 10.1038/nature12803

実験手法

X-RAY DIFFRACTION (2.595 Å)