4M20

Crystal Structure of hypothetical protein SAV0944 from Staphylococcus aureus subsp. aureus Mu50

Summary for 4M20

• Entry DOI: 10.2210/pdb4m20/pdb
• Descriptor: Uncharacterized protein, COENZYME A (3 entities in total)
• Functional Keywords: thioesterase, hot dog fold, hydrolysis of thioester bond, hydrolase
• Biological source: Staphylococcus aureus subsp. aureus
• Total number of polymer chains: 4
• Total formula weight: 56882.88

Authors

Khandokar, Y.B.,Forwood, J.K. (deposition date: 2013-08-05, release date: 2014-02-12, Last modification date: 2023-09-20)

Primary citation

Khandokar, Y.B.,Roman, N.,Smith, K.M.,Srivastava, P.,Forwood, J.K.
Expression, purification, crystallization and preliminary X-ray analysis of the PaaI-like thioesterase SAV0944 from Staphylococcus aureus.
Acta Crystallogr F Struct Biol Commun, 70:244-247, 2014

PubMed Abstract: Staphylococcus aureus is the causative agent of many diseases, including meningitis, bacteraemia, pneumonia, food poisoning and toxic shock syndrome. Structural characterization of the PaaI-like thioesterase SAV0944 (SaPaaI) from S. aureus subsp. aureus Mu50 will aid in understanding its potential as a new therapeutic target by knowledge of its molecular details and cellular functions. Here, the recombinant expression, purification and crystallization of SaPaaI thioesterase from S. aureus are reported. This protein initially crystallized with the ligand coenzyme A using the hanging-drop vapour-diffusion technique with condition No. 40 of Crystal Screen from Hampton Research at 296 K. Optimal final conditions consisting of 24% PEG 4000, 100 mM sodium citrate pH 6.5, 12% 2-propanol gave single diffraction-quality crystals. These crystals diffracted to beyond 2 Å resolution at the Australian Synchrotron and belonged to space group P12(1)1, with unit-cell parameters a = 44.05, b = 89.05, c = 60.74 Å, β = 100.5°. Initial structure determination and refinement gave an R factor and R(free) of 17.3 and 22.0%, respectively, confirming a positive solution in obtaining phases using molecular replacement.

PubMed: 24637766
DOI: 10.1107/S2053230X14000338

Experimental method

X-RAY DIFFRACTION (2 Å)