4M20

Crystal Structure of hypothetical protein SAV0944 from Staphylococcus aureus subsp. aureus Mu50

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	44.050, 89.050, 60.740
Unit cell angles	90.00, 100.53, 90.00

Refinement procedure

Resolution	30.145 - 2.000
R-factor	0.1759
Rwork	0.173
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sbk
RMSD bond length	0.009
RMSD bond angle	1.298
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.145	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	30924
Completeness [%]	99.1	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	24%PEG4000, 100mM sodium citrate pH6.5, and 12%2-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 296K