4LRM

EGFR D770_N771insNPG in complex with PD168393

4LRM の概要

• エントリーDOI: 10.2210/pdb4lrm/pdb
• 関連するPDBエントリー: 2J5F 4LL0 4LQM
• 分子名称: Epidermal growth factor receptor, N-{4-[(3-bromophenyl)amino]quinazolin-6-yl}propanamide (3 entities in total)
• 機能のキーワード: egfr, kinase, pd168393, 34-jab, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted: P00533
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 191359.73

構造登録者

Yun, C.H.,Eck, M.J. (登録日: 2013-07-20, 公開日: 2014-01-15, 最終更新日: 2023-09-20)

主引用文献

Yasuda, H.,Park, E.,Yun, C.H.,Sng, N.J.,Lucena-Araujo, A.R.,Yeo, W.L.,Huberman, M.S.,Cohen, D.W.,Nakayama, S.,Ishioka, K.,Yamaguchi, N.,Hanna, M.,Oxnard, G.R.,Lathan, C.S.,Moran, T.,Sequist, L.V.,Chaft, J.E.,Riely, G.J.,Arcila, M.E.,Soo, R.A.,Meyerson, M.,Eck, M.J.,Kobayashi, S.S.,Costa, D.B.
Structural, Biochemical, and Clinical Characterization of Epidermal Growth Factor Receptor (EGFR) Exon 20 Insertion Mutations in Lung Cancer.
Sci Transl Med, 5:216ra177-216ra177, 2013

PubMed Abstract: Epidermal growth factor receptor (EGFR) gene mutations (G719X, exon 19 deletions/insertions, L858R, and L861Q) predict favorable responses to EGFR tyrosine kinase inhibitors (TKIs) in advanced non-small cell lung cancer (NSCLC). However, EGFR exon 20 insertion mutations (~10% of all EGFR mutations) are generally associated with insensitivity to available TKIs (gefitinib, erlotinib, and afatinib). The basis of this primary resistance is poorly understood. We studied a broad subset of exon 20 insertion mutations, comparing in vitro TKI sensitivity with responses to gefitinib and erlotinib in NSCLC patients, and found that most are resistant to EGFR TKIs. The crystal structure of a representative TKI-insensitive mutant (D770_N771insNPG) reveals an unaltered adenosine triphosphate-binding pocket, and the inserted residues form a wedge at the end of the C helix that promotes the active kinase conformation. Unlike EGFR-L858R, D770_N771insNPG activates EGFR without increasing its affinity for EGFR TKIs. Unexpectedly, we find that EGFR-A763_Y764insFQEA is highly sensitive to EGFR TKIs in vitro, and patients whose NSCLCs harbor this mutation respond to erlotinib. Analysis of the A763_Y764insFQEA mutant indicates that the inserted residues shift the register of the C helix in the N-terminal direction, altering the structure in the region that is also affected by the TKI-sensitive EGFR-L858R. Our studies reveal intricate differences between EGFR mutations, their biology, and their response to EGFR TKIs.

PubMed: 24353160
DOI: 10.1126/scitranslmed.3007205

実験手法

X-RAY DIFFRACTION (3.526 Å)