4LHU

Crystal Structure of 9C2 TCR bound to CD1d

Summary for 4LHU

• Entry DOI: 10.2210/pdb4lhu/pdb
• Related: 4LFH
• Descriptor: Antigen-presenting glycoprotein CD1d, (15Z)-N-[(2S,3S,4R)-1-(alpha-D-galactopyranosyloxy)-3,4-dihydroxyoctadecan-2-yl]tetracos-15-enamide, CHLORIDE ION, ... (12 entities in total)
• Functional Keywords: nkt cells, gammadelta tcr, cd1d, lipid recognition, pbs-44, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 101305.18

Authors

Uldrich, A.P.,Le Nours, J.,Pellicci, D.G.,Gras, S.,Rossjohn, J.,Godfrey, D.I. (deposition date: 2013-07-01, release date: 2013-10-02, Last modification date: 2024-11-27)

Primary citation

Uldrich, A.P.,Le Nours, J.,Pellicci, D.G.,Gherardin, N.A.,McPherson, K.G.,Lim, R.T.,Patel, O.,Beddoe, T.,Gras, S.,Rossjohn, J.,Godfrey, D.I.
CD1d-lipid antigen recognition by the gamma delta TCR.
Nat.Immunol., 14:1137-1145, 2013

PubMed Abstract: The T cell repertoire comprises αβ and γδ T cell lineages. Although it is established how αβ T cell antigen receptors (TCRs) interact with antigen presented by antigen-presenting molecules, this is unknown for γδ TCRs. We describe a population of human Vδ1(+) γδ T cells that exhibit autoreactivity to CD1d and provide a molecular basis for how a γδ TCR binds CD1d-α-galactosylceramide (α-GalCer). The γδ TCR docked orthogonally, over the A' pocket of CD1d, in which the Vδ1-chain, and in particular the germ line-encoded CDR1δ loop, dominated interactions with CD1d. The TCR γ-chain sat peripherally to the interface, with the CDR3γ loop representing the principal determinant for α-GalCer specificity. Accordingly, we provide insight into how a γδ TCR binds specifically to a lipid-loaded antigen-presenting molecule.

PubMed: 24076636
DOI: 10.1038/ni.2713

Experimental method

X-RAY DIFFRACTION (2.87 Å)