4LG8

Crystal structure of PRPF19 WD40 repeats

Summary for 4LG8

• Entry DOI: 10.2210/pdb4lg8/pdb
• Descriptor: Pre-mRNA-processing factor 19, SODIUM ION, UNKNOWN ATOM OR ION, ... (4 entities in total)
• Functional Keywords: structural genomics consortium, sgc, wd40 repeat, dna binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus : Q9UMS4
• Total number of polymer chains: 1
• Total formula weight: 39105.89

Authors

Xu, C.,Tempel, W.,He, H.,Dobrovetsky, E.,Seitova, A.,Bountra, C.,Arrowsmith, C.H.,Edwards, A.M.,Min, J.,Structural Genomics Consortium (SGC) (deposition date: 2013-06-27, release date: 2013-08-07, Last modification date: 2023-09-20)

Primary citation

Zhang, Y.,Li, Y.,Liang, X.,Zhu, Z.,Sun, H.,He, H.,Min, J.,Liao, S.,Liu, Y.
Crystal structure of the WD40 domain of human PRPF19.
Biochem. Biophys. Res. Commun., 493:1250-1253, 2017

PubMed Abstract: Human Pre-mRNA Processing factor 19 (hPRPF19) is an important component in human spliceosome machinery. hPRPF19 contains a WD40 repeats domain at its C-terminus, which is also conserved in yeast. Here we determined the crystal structure of the C-terminal WD40 repeat domain of hPRPF19 by X-ray crystallography. Our structural analysis revealed some significantly different structure features between the human and yeast Prp19 WD40 repeat domain. However, there are also conserved clusters of residues at the bottom surface of the fourth and the fifth WD40 repeats, which provides the important implication for the conserved Prp19 proteins in both human and yeast.

PubMed: 28962858
DOI: 10.1016/j.bbrc.2017.09.145

Experimental method

X-RAY DIFFRACTION (1.89 Å)