4LF0

The E142D mutant of the amidase from Geobacillus pallidus

Summary for 4LF0

• Entry DOI: 10.2210/pdb4lf0/pdb
• Related: 2PLQ 4GYL 4GYN 4KZF
• Descriptor: Aliphatic amidase (2 entities in total)
• Functional Keywords: alpha-beta-beta-alpha sandwich, hydrolase
• Biological source: Bacillus sp.
• Total number of polymer chains: 1
• Total formula weight: 38626.81

Authors

Sewell, B.T.,Weber, B.W.,Kimani, S.W.,Cowan, D.A.,Hunter, R.,Venter, G.A.,Gumbart, J.C.,Thuku, R.N.,Varsani, A. (deposition date: 2013-06-26, release date: 2013-08-21, Last modification date: 2024-03-20)

• Primary citation: Weber, B.W.,Kimani, S.W.,Varsani, A.,Cowan, D.A.,Hunter, R.,Venter, G.A.,Gumbart, J.C.,Sewell, B.T.; The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning.; J.Biol.Chem., 288:28514-28523, 2013; PubMed: 23946488; DOI: 10.1074/jbc.M113.503284

Experimental method

X-RAY DIFFRACTION (1.1 Å)