4LF0

The E142D mutant of the amidase from Geobacillus pallidus

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004040	molecular_function	amidase activity
A	0016787	molecular_function	hydrolase activity
A	0016811	molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000250

Chain	Residue	Details
A	GLU59

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000250

Chain	Residue	Details
A	LYS134

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site_id	SWS_FT_FI3
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000250

Chain	Residue	Details
A	CYS166

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