4L8R

Structure of mrna stem-loop, human stem-loop binding protein and 3'hexo ternary complex

Replaces:  4HXH

Summary for 4L8R

• Entry DOI: 10.2210/pdb4l8r/pdb
• Descriptor: HISTONE MRNA STEM-LOOP, 3'-5' exoribonuclease 1, Histone RNA hairpin-binding protein, ... (4 entities in total)
• Functional Keywords: rna-rna binding protein-hydrolase complex, histone mrna 3'-end processing, histone mrna translation, microrna homeostasis, 5.8s rrna 3 -end maturation, zfp100, lsm11, nucleus, rna/rna binding protein/hydrolase
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm : Q8IV48 Q14493
• Total number of polymer chains: 5
• Total formula weight: 102149.97

Authors

Tan, D.,Tong, L. (deposition date: 2013-06-17, release date: 2013-07-10, Last modification date: 2024-11-27)

Primary citation

Tan, D.,Marzluff, W.F.,Dominski, Z.,Tong, L.
Structure of Histone Mrna Stem-Loop, Human Stem-Loop Binding Protein, and 3'Hexo Ternary Complex.
Science, 339:318-, 2013

PubMed Abstract: Metazoan replication-dependent histone messenger RNAs (mRNAs) have a conserved stem-loop (SL) at their 3'-end. The stem-loop binding protein (SLBP) specifically recognizes the SL to regulate histone mRNA metabolism, and the 3'-5' exonuclease 3'hExo trims its 3'-end after processing. We report the crystal structure of a ternary complex of human SLBP RNA binding domain, human 3'hExo, and a 26-nucleotide SL RNA. Only one base of the SL is recognized specifically by SLBP, and the two proteins primarily recognize the shape of the RNA. SLBP and 3'hExo have no direct contact with each other, and induced structural changes in the loop of the SL mediate their cooperative binding. The 3' flanking sequence is positioned in the 3'hExo active site, but the ternary complex limits the extent of trimming.

PubMed: 23329046
DOI: 10.1126/SCIENCE.1228705

Experimental method

X-RAY DIFFRACTION (2.6 Å)