4L8R

Structure of mrna stem-loop, human stem-loop binding protein and 3'hexo ternary complex

「4HXH」から置き換えられました

4L8R の概要

• エントリーDOI: 10.2210/pdb4l8r/pdb
• 分子名称: HISTONE MRNA STEM-LOOP, 3'-5' exoribonuclease 1, Histone RNA hairpin-binding protein, ... (4 entities in total)
• 機能のキーワード: rna-rna binding protein-hydrolase complex, histone mrna 3'-end processing, histone mrna translation, microrna homeostasis, 5.8s rrna 3 -end maturation, zfp100, lsm11, nucleus, rna/rna binding protein/hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm : Q8IV48 Q14493
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 102149.97

構造登録者

Tan, D.,Tong, L. (登録日: 2013-06-17, 公開日: 2013-07-10, 最終更新日: 2017-11-15)

主引用文献

Tan, D.,Marzluff, W.F.,Dominski, Z.,Tong, L.
Structure of Histone Mrna Stem-Loop, Human Stem-Loop Binding Protein, and 3'Hexo Ternary Complex.
Science, 339:318-, 2013

PubMed Abstract: Metazoan replication-dependent histone messenger RNAs (mRNAs) have a conserved stem-loop (SL) at their 3'-end. The stem-loop binding protein (SLBP) specifically recognizes the SL to regulate histone mRNA metabolism, and the 3'-5' exonuclease 3'hExo trims its 3'-end after processing. We report the crystal structure of a ternary complex of human SLBP RNA binding domain, human 3'hExo, and a 26-nucleotide SL RNA. Only one base of the SL is recognized specifically by SLBP, and the two proteins primarily recognize the shape of the RNA. SLBP and 3'hExo have no direct contact with each other, and induced structural changes in the loop of the SL mediate their cooperative binding. The 3' flanking sequence is positioned in the 3'hExo active site, but the ternary complex limits the extent of trimming.

PubMed: 23329046
DOI: 10.1126/SCIENCE.1228705

実験手法

X-RAY DIFFRACTION (2.6 Å)