4L01

Crystal structure of the V658F apo Jak1 pseudokinase domain

4L01 の概要

• エントリーDOI: 10.2210/pdb4l01/pdb
• 関連するPDBエントリー: 4L00
• 分子名称: Tyrosine-protein kinase JAK1 (2 entities in total)
• 機能のキーワード: kinase domain fold, regulatory, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Endomembrane system; Peripheral membrane protein: P23458
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69932.32

構造登録者

Toms, A.V.,Rogers, J.M.,Eck, M.J. (登録日: 2013-05-30, 公開日: 2013-09-04, 最終更新日: 2024-02-28)

主引用文献

Toms, A.V.,Deshpande, A.,McNally, R.,Jeong, Y.,Rogers, J.M.,Kim, C.U.,Gruner, S.M.,Ficarro, S.B.,Marto, J.A.,Sattler, M.,Griffin, J.D.,Eck, M.J.
Structure of a pseudokinase-domain switch that controls oncogenic activation of Jak kinases.
Nat.Struct.Mol.Biol., 20:1221-1223, 2013

PubMed Abstract: The V617F mutation in the Jak2 pseudokinase domain causes myeloproliferative neoplasms, and the equivalent mutation in Jak1 (V658F) is found in T-cell leukemias. Crystal structures of wild-type and V658F-mutant human Jak1 pseudokinase reveal a conformational switch that remodels a linker segment encoded by exon 12, which is also a site of mutations in Jak2. This switch is required for V617F-mediated Jak2 activation and possibly for physiologic Jak activation.

PubMed: 24013208
DOI: 10.1038/nsmb.2673

実験手法

X-RAY DIFFRACTION (1.9 Å)