4KGC

Nucleosome Core Particle Containing (ETA6-P-CYMENE)-(1, 2-ETHYLENEDIAMINE)-RUTHENIUM

Summary for 4KGC

• Entry DOI: 10.2210/pdb4kgc/pdb
• Descriptor: Histone H3.2, Histone H4, Histone H2A, ... (9 entities in total)
• Functional Keywords: dna-protein complex, nucleosome, ruthenium agents, structural protein-dna complex, structural protein/dna
• Biological source: Xenopus laevis (clawed frog,common platanna,platanna); More
• Cellular location: Nucleus: P84233 P62799 P02281; Chromosome . Nucleus : Q6AZJ8
• Total number of polymer chains: 10
• Total formula weight: 200792.86

Authors

Adhireksan, Z.,Davey, C.A. (deposition date: 2013-04-29, release date: 2014-03-26, Last modification date: 2024-03-20)

Primary citation

Adhireksan, Z.,Davey, G.E.,Campomanes, P.,Groessl, M.,Clavel, C.M.,Yu, H.,Nazarov, A.A.,Yeo, C.H.,Ang, W.H.,Droge, P.,Rothlisberger, U.,Dyson, P.J.,Davey, C.A.
Ligand substitutions between ruthenium-cymene compounds can control protein versus DNA targeting and anticancer activity
Nat Commun, 5:3462-3462, 2014

PubMed Abstract: Ruthenium compounds have become promising alternatives to platinum drugs by displaying specific activities against different cancers and favourable toxicity and clearance properties. Nonetheless, their molecular targeting and mechanism of action are poorly understood. Here we study two prototypical ruthenium-arene agents-the cytotoxic antiprimary tumour compound [(η(6)-p-cymene)Ru(ethylene-diamine)Cl]PF6 and the relatively non-cytotoxic antimetastasis compound [(η(6)-p-cymene)Ru(1,3,5-triaza-7-phosphaadamantane)Cl2]-and discover that the former targets the DNA of chromatin, while the latter preferentially forms adducts on the histone proteins. Using a novel 'atom-to-cell' approach, we establish the basis for the surprisingly site-selective adduct formation behaviour and distinct cellular impact of these two chemically similar anticancer agents, which suggests that the cytotoxic effects arise largely from DNA lesions, whereas the protein adducts may be linked to the other therapeutic activities. Our study shows promise for developing new ruthenium drugs, via ligand-based modulation of DNA versus protein binding and thus cytotoxic potential, to target distinguishing epigenetic features of cancer cells.

PubMed: 24637564
DOI: 10.1038/ncomms4462

Experimental method

X-RAY DIFFRACTION (2.69 Å)