4KGC

Nucleosome Core Particle Containing (ETA6-P-CYMENE)-(1, 2-ETHYLENEDIAMINE)-RUTHENIUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	90
Detector technology	CCD
Collection date	2011-07-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.50
Spacegroup name	P 21 21 21
Unit cell lengths	106.620, 109.710, 181.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.600 - 2.690
R-factor	0.24835
Rwork	0.248
R-free	0.28234
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.432
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.600	2.840
High resolution limit [Å]	2.690	2.690
Number of reflections	55781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	40 mM MnCl2, 30 mM KCl, 20 mM K-Cacodylate , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K