4KFB

HIV-1 reverse transcriptase with bound fragment at NNRTI adjacent site

「4I7G」から置き換えられました

4KFB の概要

• エントリーDOI: 10.2210/pdb4kfb/pdb
• 分子名称: REVERSE TRANSCRIPTASE/RIBONUCLEASE H, EXORIBONUCLEA P66 RT, P51 RT, ethyl pyrazolo[1,5-a]pyridine-3-carboxylate, ... (6 entities in total)
• 機能のキーワード: rna-directed dna polymerase, dna polymerase, endonuclease, hydrolase, multifunctional enzyme, hydrolase-inhibitor complex, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1); 詳細
• 細胞内の位置: Gag-Pol polyprotein: Host cell membrane; Lipid-anchor. Matrix protein p17: Virion membrane; Lipid- anchor . Capsid protein p24: Virion . Nucleocapsid protein p7: Virion . Reverse transcriptase/ribonuclease H: Virion . Integrase: Virion : P03366 P03366
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 115423.72

構造登録者

Bauman, J.D.,Patel, D.,Arnold, E. (登録日: 2013-04-26, 公開日: 2013-05-15, 最終更新日: 2024-02-28)

主引用文献

Bauman, J.D.,Patel, D.,Dharia, C.,Fromer, M.W.,Ahmed, S.,Frenkel, Y.,Vijayan, R.S.,Eck, J.T.,Ho, W.C.,Das, K.,Shatkin, A.J.,Arnold, E.
Detecting Allosteric Sites of HIV-1 Reverse Transcriptase by X-Ray Crystallographic Fragment Screening.
J.Med.Chem., 56:2738-, 2013

PubMed Abstract: HIV-1 reverse transcriptase (RT) undergoes a series of conformational changes during viral replication and is a central target for antiretroviral therapy. The intrinsic flexibility of RT can provide novel allosteric sites for inhibition. Crystals of RT that diffract X-rays to better than 2 Å resolution facilitated the probing of RT for new druggable sites using fragment screening by X-ray crystallography. A total of 775 fragments were grouped into 143 cocktails, which were soaked into crystals of RT in complex with the non-nucleoside drug rilpivirine (TMC278). Seven new sites were discovered, including the Incoming Nucleotide Binding, Knuckles, NNRTI Adjacent, and 399 sites, located in the polymerase region of RT, and the 428, RNase H Primer Grip Adjacent, and 507 sites, located in the RNase H region. Three of these sites (Knuckles, NNRTI Adjacent, and Incoming Nucleotide Binding) are inhibitory and provide opportunities for discovery of new anti-AIDS drugs.

PubMed: 23342998
DOI: 10.1021/JM301271J

実験手法

X-RAY DIFFRACTION (1.854 Å)