4JQW

Crystal Structure of a Complex of NOD1 CARD and Ubiquitin

Summary for 4JQW

• Entry DOI: 10.2210/pdb4jqw/pdb
• Related: 1AAR 1UBQ 2NSN 2NZ7 2O6V 4E9M
• Descriptor: Nucleotide-binding oligomerization domain-containing protein 1, Polyubiquitin-C, PHOSPHATE ION (3 entities in total)
• Functional Keywords: death domain-like fold, innate immunity, rip2, atg16l, s-dimethylarsenic, apoptosis-signalling protein complex, apoptosis/signalling protein
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm: Q9Y239; Ubiquitin: Cytoplasm : P0CG48
• Total number of polymer chains: 2
• Total formula weight: 20713.42

Authors

Ver Heul, A.M.,Gakhar, L.,Piper, R.C.,Ramaswamy, S. (deposition date: 2013-03-20, release date: 2014-03-26, Last modification date: 2024-10-16)

Primary citation

Ver Heul, A.M.,Gakhar, L.,Piper, R.C.,Ramaswamy, S.
Crystal structure of a complex of NOD1 CARD and ubiquitin
Plos One, 9:e104017-e104017, 2014

PubMed Abstract: The Caspase Recruitment Domain (CARD) from the innate immune receptor NOD1 was crystallized with Ubiquitin (Ub). NOD1 CARD was present as a helix-swapped homodimer similar to other structures of NOD1 CARD, and Ub monomers formed a homodimer similar in conformation to Lys48-linked di-Ub. The interaction between NOD1 CARD and Ub in the crystal was mediated by novel binding sites on each molecule. Comparisons of these sites to previously identified interaction surfaces on both molecules were made along with discussion of their potential functional significance.

PubMed: 25127239
DOI: 10.1371/journal.pone.0104017

Experimental method

X-RAY DIFFRACTION (2.9 Å)