4JQW
Crystal Structure of a Complex of NOD1 CARD and Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-23 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.688, 61.688, 86.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.982 - 2.900 |
| R-factor | 0.2302 |
| Rwork | 0.226 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 2NSN 1ubq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.704 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.982 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.050 | 0.207 |
| Number of reflections | 4048 | |
| <I/σ(I)> | 28.4 | 11.4 |
| Completeness [%] | 99.9 | |
| Redundancy | 13.35 | 13.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 291 | 100mM PCB, 25% PEG1500, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
