4JL3
Crystal structure of ms6564-dna complex
Summary for 4JL3
| Entry DOI | 10.2210/pdb4jl3/pdb |
| Related | 4JKZ |
| Descriptor | Transcriptional regulator, TetR family, DNA (31-MER), ... (4 entities in total) |
| Functional Keywords | transcription regulator, transcription-dna complex, transcription/dna |
| Biological source | Mycobacterium smegmatis |
| Total number of polymer chains | 6 |
| Total formula weight | 105110.41 |
| Authors | Yang, S.F.,Gao, Z.Q.,He, Z.G.,Dong, Y.H. (deposition date: 2013-03-12, release date: 2013-06-26, Last modification date: 2024-11-27) |
| Primary citation | Yang, S.,Gao, Z.,Li, T.,Yang, M.,Zhang, T.,Dong, Y.,He, Z.G. Structural basis for interaction between Mycobacterium smegmatis Ms6564, a TetR family master regulator, and its target DNA. J.Biol.Chem., 288:23687-23695, 2013 Cited by PubMed Abstract: Master regulators, which broadly affect expression of diverse genes, play critical roles in bacterial growth and environmental adaptation. However, the underlying mechanism by which such regulators interact with their cognate DNA remains to be elucidated. In this study, we solved the crystal structure of a broad regulator Ms6564 in Mycobacterium smegmatis and its protein-operator complex at resolutions of 1.9 and 2.5 Å, respectively. Similar to other typical TetR family regulators, two dimeric Ms6564 molecules were found to bind to opposite sides of target DNA. However, the recognition helix of Ms6564 inserted only slightly into the DNA major groove. Unexpectedly, 11 disordered water molecules bridged the interface of TetR family regulator DNA. Although the DNA was deformed upon Ms6564 binding, it still retained the conformation of B-form DNA. Within the DNA-binding domain of Ms6564, only two amino acids residues directly interacted with the bases of cognate DNA. Lys-47 was found to be essential for the specific DNA binding ability of Ms6564. These data indicate that Ms6564 can bind DNA with strong affinity but makes flexible contacts with DNA. Our study suggests that Ms6564 might slide more easily along the genomic DNA and extensively regulate the expression of diverse genes in M. smegmatis. PubMed: 23803605DOI: 10.1074/jbc.M113.468694 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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