4JL3
Crystal structure of ms6564-dna complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 |
Unit cell lengths | 100.954, 100.954, 99.856 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.497 - 2.500 |
R-factor | 0.2241 |
Rwork | 0.224 |
R-free | 0.27970 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.317 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.112 | 0.610 |
Number of reflections | 39254 | |
<I/σ(I)> | 34.7 | 2.37 |
Completeness [%] | 100.0 | 95.1 |
Redundancy | 6.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 10% PEG3000, 100mM imidazole, 200mM lithium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |