4JE3

An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation

Summary for 4JE3

• Entry DOI: 10.2210/pdb4je3/pdb
• Related: 4IT3
• Descriptor: Central kinetochore subunit IML3, Central kinetochore subunit CHL4 (3 entities in total)
• Functional Keywords: kinetochore, beta sheet, chromosome segregation, iml3-chl4 dimer, nucleus, cell cycle
• Biological source: Saccharomyces cerevisiae (Baker's yeast); More
• Cellular location: Nucleus: P38265 P38907
• Total number of polymer chains: 2
• Total formula weight: 39089.67

Authors

Hinshaw, S.M.,Harrison, S.C. (deposition date: 2013-02-26, release date: 2013-10-16, Last modification date: 2024-02-28)

Primary citation

Hinshaw, S.M.,Harrison, S.C.
An iml3-chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation.
Cell Rep, 5:29-36, 2013

PubMed Abstract: Accurate segregation of genetic material in eukaryotes relies on the kinetochore, a multiprotein complex that connects centromeric DNA with microtubules. In yeast and humans, two proteins-Mif2/CENP-C and Chl4/CNEP-N-interact with specialized centromeric nucleosomes and establish distinct but cross-connecting axes of chromatin-microtubule linkage. Proteins recruited by Chl4/CENP-N include a subset that regulates chromosome transmission fidelity. We show that Chl4 and a conserved member of this subset, Iml3, both from Saccharomyces cerevisiae, form a stable protein complex that interacts with Mif2 and Sgo1. We have determined the structures of an Iml3 homodimer and an Iml3-Chl4 heterodimer, which suggest a mechanism for regulating the assembly of this functional axis of the kinetochore. We propose that at the core centromere, the Chl4-Iml3 complex participates in recruiting factors, such as Sgo1, that influence sister chromatid cohesion and encourage sister kinetochore biorientation.

PubMed: 24075991
DOI: 10.1016/j.celrep.2013.08.036

Experimental method

X-RAY DIFFRACTION (2.282 Å)