4JE3
An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-02-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 38.483, 143.916, 146.909 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.000 - 2.282 |
R-factor | 0.1855 |
Rwork | 0.183 |
R-free | 0.21060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.175 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.370 |
High resolution limit [Å] | 2.282 | 4.930 | 2.282 |
Rmerge | 0.076 | 0.045 | 0.453 |
Number of reflections | 19061 | ||
<I/σ(I)> | 10.1 | ||
Completeness [%] | 98.9 | 100 | 91.4 |
Redundancy | 7.1 | 6.6 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 0.35 M lithium citrate, pH 9, 25% w/v PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |