4IYE

Crystal structure of AdTx1 (rho-Da1a) from eastern green mamba (Dendroaspis angusticeps)

4IYE の概要

• エントリーDOI: 10.2210/pdb4iye/pdb
• 関連するPDBエントリー: 1FF4 2H5F 2H7Z 2VLW 3FEV 3NEQ 4DO8
• 分子名称: Toxin AdTx1, 1,2-ETHANEDIOL, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: snake three-finger toxin family, type a muscarinic toxin subfamily, allosteric antagonist of the alpha-1a adrenergic receptor (adra1a), acts as a relaxant of smooth muscle, alpha-1a adrenergic receptor, g-rhoda1a k34a, expressed by the venom gland, toxin
• 由来する生物種: Dendroaspis angusticeps (Eastern green mamba)
• 細胞内の位置: Secreted: P85092
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7574.58

構造登録者

Stura, E.A.,Vera, L.,Maiga, A.A.,Marchetti, C.,Lorphelin, A.,Bellanger, L.,Servant, D.,Gilles, N. (登録日: 2013-01-28, 公開日: 2013-05-08, 最終更新日: 2024-11-27)

主引用文献

Maiga, A.,Vera, L.,Marchetti, C.,Lorphelin, A.,Bellanger, L.,Mourier, G.,Servent, D.,Gilles, N.,Stura, E.A.
Crystallization of recombinant green mamba rho-Da1a toxin during a lyophilization procedure and its structure determination.
Acta Crystallogr.,Sect.F, 69:704-709, 2013

PubMed Abstract: ρ-Da1a toxin from eastern green mamba (Dendroaspis angusticeps) venom is a polypeptide of 65 amino acids with a strong affinity for the G-protein-coupled α(1A)-adrenoceptor. This neurotoxin has been crystallized from resolubilized lyophilized powder, but the best crystals grew spontaneously during lyophilization. The crystals belonged to the trigonal space group P3(1)21, with unit-cell parameters a = b = 37.37, c = 66.05 Å, and diffracted to 1.95 Å resolution. The structure solved by molecular replacement showed strong similarities to green mamba muscarinic toxins.

PubMed: 23722859
DOI: 10.1107/S1744309113011470

実験手法

X-RAY DIFFRACTION (1.951 Å)