4IYE

Crystal structure of AdTx1 (rho-Da1a) from eastern green mamba (Dendroaspis angusticeps)

Summary for 4IYE

• Entry DOI: 10.2210/pdb4iye/pdb
• Related: 1FF4 2H5F 2H7Z 2VLW 3FEV 3NEQ 4DO8
• Descriptor: Toxin AdTx1, 1,2-ETHANEDIOL, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• Functional Keywords: snake three-finger toxin family, type a muscarinic toxin subfamily, allosteric antagonist of the alpha-1a adrenergic receptor (adra1a), acts as a relaxant of smooth muscle, alpha-1a adrenergic receptor, g-rhoda1a k34a, expressed by the venom gland, toxin
• Biological source: Dendroaspis angusticeps (Eastern green mamba)
• Cellular location: Secreted: P85092
• Total number of polymer chains: 1
• Total formula weight: 7574.58

Authors

Stura, E.A.,Vera, L.,Maiga, A.A.,Marchetti, C.,Lorphelin, A.,Bellanger, L.,Servant, D.,Gilles, N. (deposition date: 2013-01-28, release date: 2013-05-08, Last modification date: 2024-11-27)

Primary citation

Maiga, A.,Vera, L.,Marchetti, C.,Lorphelin, A.,Bellanger, L.,Mourier, G.,Servent, D.,Gilles, N.,Stura, E.A.
Crystallization of recombinant green mamba rho-Da1a toxin during a lyophilization procedure and its structure determination.
Acta Crystallogr.,Sect.F, 69:704-709, 2013

PubMed Abstract: ρ-Da1a toxin from eastern green mamba (Dendroaspis angusticeps) venom is a polypeptide of 65 amino acids with a strong affinity for the G-protein-coupled α(1A)-adrenoceptor. This neurotoxin has been crystallized from resolubilized lyophilized powder, but the best crystals grew spontaneously during lyophilization. The crystals belonged to the trigonal space group P3(1)21, with unit-cell parameters a = b = 37.37, c = 66.05 Å, and diffracted to 1.95 Å resolution. The structure solved by molecular replacement showed strong similarities to green mamba muscarinic toxins.

PubMed: 23722859
DOI: 10.1107/S1744309113011470

Experimental method

X-RAY DIFFRACTION (1.951 Å)