4I79

Crystal structure of human NUP43

Summary for 4I79

• Entry DOI: 10.2210/pdb4i79/pdb
• Descriptor: Nucleoporin Nup43, floating chain, unknown sequence, UNKNOWN ATOM OR ION, ... (4 entities in total)
• Functional Keywords: structural genomics consortium, sgc, wd40 repeat, cell cycle
• Biological source: Homo sapiens (human); More
• Cellular location: Chromosome, centromere, kinetochore: Q8NFH3
• Total number of polymer chains: 3
• Total formula weight: 89241.68

Authors

Xu, C.,Tempel, W.,Li, Z.,He, H.,Wernimont, A.K.,Bountra, C.,Arrowsmith, C.H.,Edwards, A.M.,Min, J.,Structural Genomics Consortium (SGC) (deposition date: 2012-11-30, release date: 2013-02-27, Last modification date: 2023-09-20)

Primary citation

Xu, C.,Li, Z.,He, H.,Seitova, A.,Wernimont, A.,Li, Y.,Loppnau, P.,Min, J.
Crystal structure of human nuclear pore complex component NUP43.
Febs Lett., 589:3247-3253, 2015

PubMed Abstract: Nuclear pore complexes (NPC) form nuclear pores that cross the nuclear envelope and allow molecules to transport between the nucleus and the cytoplasm. We solved the crystal structure of human Nup43 (hNUP43), an important component in the Nup107 subcomplex of NPC. hNup43 adopts a seven-bladed β-propeller fold. We confirmed by ITC that neither human Nup37 (hNup37) nor human Nup133 (hNup133) interacts with hNup43. We demonstrated by analytical gel filtration that the human Nup85-Seh1L binary complex recruits hNup43 to form a ternary complex. Based on amino acid sequence analysis, we predicted the hNup85-hSeh1L binding surface of hNup43.

PubMed: 26391640
DOI: 10.1016/j.febslet.2015.09.008

Experimental method

X-RAY DIFFRACTION (1.75 Å)