4I79

Crystal structure of human NUP43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-20
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 2
Unit cell lengths	80.736, 161.964, 58.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 1.750
R-factor	0.1821
Rwork	0.181
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3cfs 4aow 2xzm.
RMSD bond length	0.016
RMSD bond angle	1.533
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.982	47.210	1.840
High resolution limit [Å]	1.600	5.530	1.750
Rmerge		0.033	0.940
Total number of observations		18204	83285
Number of reflections	77846
<I/σ(I)>	15.8	12.5	0.8
Completeness [%]	100.0	99.8	100
Redundancy	7.4	6.7	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	25% PEG-3350, 0.1 M ammonium sulfate, 0.1 M sodium Hepes, pH 7.5, VAPOR DIFFUSION, temperature 291K