4I5Z

Insulin protein crystallization via langmuir-blodgett

4I5Z の概要

• エントリーDOI: 10.2210/pdb4i5z/pdb
• 関連するPDBエントリー: 2BN1 4I5Y
• 分子名称: Insulin (3 entities in total)
• 機能のキーワード: thin films, optimal crystallization, space grown, langmuir blodgett, hormone
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow); 詳細
• 細胞内の位置: Secreted: P01317 P01317
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5743.57

構造登録者

Belmonte, L.,Pechkova, E.,Bragazzi, N.,Nicolini, C. (登録日: 2012-11-29, 公開日: 2013-11-20, 最終更新日: 2024-10-30)

主引用文献

Pechkova, E.,Bragazzi, N.,Bozdaganyan, M.,Belmonte, L.,Nicolini, C.
A review of the strategies for obtaining high-quality crystals utilizing nanotechnologies and microgravity
Crit Rev Eukaryot Gene Expr, 24:325-339, 2014

PubMed Abstract: Crystallization is a highly demanding and time-consuming task that causes a real bottle-neck in basic research. Great effort has been made to understand the factors and parameters that influence this process and to finely tune them to facilitate crystal growth. Different crystallization techniques have been proposed over the past decades, such as the classical vapor hanging drop method, its variant the sitting drop method, dialysis, cryo-temperature, gel, batch, and the innovative microgravity (space) techniques like free interface diffusion (FID) and counter-ion diffusion (CID). Here, we present a review of the strategies utilizing Langmuir-Blodgett (LB)-based nanotechnologies, and microgravity techniques for obtaining optimal high-quality crystals, as proven by molecular dynamics (MD) and bioinformatics approaches, namely using a clustering algorithm and protein alignment.

PubMed: 25403962
DOI: 10.1615/CritRevEukaryotGeneExpr.2014008275

実験手法

X-RAY DIFFRACTION (1.8 Å)