4HVZ

Crystal structure of brucella abortus immunogenic BP26 protein

Summary for 4HVZ

• Entry DOI: 10.2210/pdb4hvz/pdb
• Descriptor: 26 kDa periplasmic immunogenic protein (1 entity in total)
• Functional Keywords: multimerization, simpl domain, infection, membrane protein
• Biological source: Brucella abortus
• Cellular location: Periplasm: Q44642
• Total number of polymer chains: 4
• Total formula weight: 95208.38

Authors

Kim, D.,Park, J.,Oh, B.,Song, J. (deposition date: 2012-11-07, release date: 2013-01-30, Last modification date: 2024-02-28)

Primary citation

Kim, D.,Park, J.,Kim, S.J.,Soh, Y.M.,Kim, H.M.,Oh, B.H.,Song, J.J.
Brucella Immunogenic BP26 Forms a Channel-like Structure.
J.Mol.Biol., 425:1119-1126, 2013

PubMed Abstract: An outer membrane protein BP26/OMP28 of Brucella, BP26, is identified as a major immunodominant antigen and widely used as a diagnostic marker and for vaccination against Brucellosis. BP26 belongs to the family of proteins that contains a SIMPL (signaling molecule that associates with the mouse pelle-like kinase) domain, whose structure and function have been unknown. Here, we present the crystal structure of BP26 revealing that 16 BP26 molecules form a novel channel-like assembly as also shown by electron microscopy analysis. Eight BP26 molecules forming a ring structure contain a hole at the center of the octamer, and another octamer interacts with each other to form a channel having a large internal cavity. BP26 is found to be structurally similar to a bacteriophage protein involved in infection, implicating that BP26 might function during Brucella infection. In addition, the BP26 structure suggests that the protein functions as a multimeric channel-like form and provides a canonical model for the SIMPL domains.

PubMed: 23353825
DOI: 10.1016/j.jmb.2013.01.015

Experimental method

X-RAY DIFFRACTION (3.5 Å)