4HVZ

Crystal structure of brucella abortus immunogenic BP26 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Temperature [K]	110
Detector technology	CCD
Collection date	2012-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97941
Spacegroup name	I 41 2 2
Unit cell lengths	203.086, 203.086, 207.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.960 - 3.500
Rwork	0.237
R-free	0.28300
Structure solution method	SAD
RMSD bond length	0.024
RMSD bond angle	2.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.560
High resolution limit [Å]	3.500	3.500
Rmerge		0.531
Number of reflections	26996
<I/σ(I)>	31.9	3.8
Completeness [%]		99.3
Redundancy	5.9	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	2.4M AMS, 100mM NaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K