4HHJ

Dengue serotype 3 RNA-dependent RNA polymerase

Summary for 4HHJ

• Entry DOI: 10.2210/pdb4hhj/pdb
• Related: 3VWS
• Descriptor: Non-structural protein 5, ZINC ION, HEXAETHYLENE GLYCOL, ... (5 entities in total)
• Functional Keywords: rdrp, viral protein, rna-dependent rna polymerase, rna binding, er membrane, transferase
• Biological source: Dengue virus 3
• Cellular location: Envelope protein E: Virion membrane; Multi- pass membrane protein: Q6DLV0
• Total number of polymer chains: 1
• Total formula weight: 74356.38

Authors

Noble, C.G.,Lescar, J. (deposition date: 2012-10-10, release date: 2013-02-27, Last modification date: 2023-11-08)

Primary citation

Noble, C.G.,Lim, S.P.,Chen, Y.L.,Liew, C.W.,Yap, L.,Lescar, J.,Shi, P.-Y.
Conformational flexibility of the Dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor
J.Virol., 87:5291-5295, 2013

PubMed Abstract: We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype 3 (DENV-3), allowing structure refinement to a 1.79-Å resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor cocrystal structure at a 2.1-Å resolution. The inhibitor binds to the RdRp as a dimer and causes conformational changes in the protein. The improved crystallization conditions and new structural information should accelerate structure-based drug discovery.

PubMed: 23408636
DOI: 10.1128/JVI.00045-13

Experimental method

X-RAY DIFFRACTION (1.79 Å)