4HBT

Crystal structure of native CTX-M-15 extended-spectrum beta-lactamase

4HBT の概要

• エントリーDOI: 10.2210/pdb4hbt/pdb
• 関連するPDBエントリー: 4GZB 4HBU 4HEF
• 分子名称: Beta-lactamase, SULFATE ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: hydrolysis of beta-lactams, antibiotic resistance, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29151.14

構造登録者

Docquier, J.D.,Benvenuti, M.,Bruneau, J.M.,Rossolini, G.M.,Miossec, C.,Black, M.T.,Mangani, S. (登録日: 2012-09-28, 公開日: 2013-04-10, 最終更新日: 2023-11-08)

主引用文献

Lahiri, S.D.,Mangani, S.,Durand-Reville, T.,Benvenuti, M.,De Luca, F.,Sanyal, G.,Docquier, J.D.
Structural insight into potent broad-spectrum inhibition with reversible recyclization mechanism: avibactam in complex with CTX-M-15 and Pseudomonas aeruginosa AmpC beta-lactamases
Antimicrob.Agents Chemother., 57:2496-2505, 2013

PubMed Abstract: Although β-lactams have been the most effective class of antibacterial agents used in clinical practice for the past half century, their effectiveness on Gram-negative bacteria has been eroded due to the emergence and spread of β-lactamase enzymes that are not affected by currently marketed β-lactam/β-lactamase inhibitor combinations. Avibactam is a novel, covalent, non-β-lactam β-lactamase inhibitor presently in clinical development in combination with either ceftaroline or ceftazidime. In vitro studies show that avibactam may restore the broad-spectrum activity of cephalosporins against class A, class C, and some class D β-lactamases. Here we describe the structures of two clinically important β-lactamase enzymes bound to avibactam, the class A CTX-M-15 extended-spectrum β-lactamase and the class C Pseudomonas aeruginosa AmpC β-lactamase, which together provide insight into the binding modes for the respective enzyme classes. The structures reveal similar binding modes in both enzymes and thus provide a rationale for the broad-spectrum inhibitory activity of avibactam. Identification of the key residues surrounding the binding pocket allows for a better understanding of the potency of this scaffold. Finally, avibactam has recently been shown to be a reversible inhibitor, and the structures provide insights into the mechanism of avibactam recyclization. Analysis of the ultra-high-resolution CTX-M-15 structure suggests how the deacylation mechanism favors recyclization over hydrolysis.

PubMed: 23439634
DOI: 10.1128/AAC.02247-12

実験手法

X-RAY DIFFRACTION (1.1 Å)