4H3K

Crystal structure of a ternary complex of human symplekin NTD, human Ssu72 and a RNA polymerase II CTD peptide phosphorylated at Ser-2, Ser-5 and Ser-7

Summary for 4H3K

• Entry DOI: 10.2210/pdb4h3k/pdb
• Related: 3O2Q 4H3H
• Descriptor: Symplekin, RNA polymerase II subunit A C-terminal domain phosphatase SSU72, Hexapeptide, ... (5 entities in total)
• Functional Keywords: heat repeat, phosphatase, rna polymerase ii, hydrolase
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm, cytoskeleton: Q92797; Nucleus: Q9NP77
• Total number of polymer chains: 5
• Total formula weight: 130221.48

Authors

Xiang, K.,Tong, L. (deposition date: 2012-09-13, release date: 2012-10-31, Last modification date: 2024-10-30)

Primary citation

Xiang, K.,Manley, J.L.,Tong, L.
An unexpected binding mode for a Pol II CTD peptide phosphorylated at Ser7 in the active site of the CTD phosphatase Ssu72.
Genes Dev., 26:2265-2270, 2012

PubMed Abstract: Ssu72, an RNA polymerase II C-terminal domain (CTD) phospho-Ser5 (pSer5) phosphatase, was recently reported to have pSer7 phosphatase activity as well. We report here the crystal structure of a ternary complex of the N-terminal domain of human symplekin, human Ssu72, and a 10-mer pSer7 CTD peptide. Surprisingly, the peptide is bound in the Ssu72 active site with its backbone running in the opposite direction compared with a pSer5 peptide. The pSer7 phosphatase activity of Ssu72 is ∼4000-fold lower than its pSer5 phosphatase activity toward a peptide substrate, consistent with the structural observations.

PubMed: 23070812
DOI: 10.1101/gad.198853.112

Experimental method

X-RAY DIFFRACTION (2 Å)