Summary for 4H1P
| Entry DOI | 10.2210/pdb4h1p/pdb |
| Related | 1H87 |
| Descriptor | Lysozyme C, EUROPIUM ION, CHLORIDE ION, ... (5 entities in total) |
| Functional Keywords | hewl, lysozyme activity, catalytic activity, hydrolase |
| Biological source | Gallus gallus (bantam,chickens) |
| Total number of polymer chains | 1 |
| Total formula weight | 15154.42 |
| Authors | Vijayakumar, B.,Velmurugan, D. (deposition date: 2012-09-11, release date: 2012-10-24, Last modification date: 2024-11-20) |
| Primary citation | Vijayakumar, B.,Velmurugan, D. Use of europium ions for SAD phasing of Lysozyme at the Cu Kalpha wavelength Acta Crystallogr.,Sect.F, 69:20-24, 2013 Cited by PubMed Abstract: Europium is shown to be a good anomalous scatterer in SAD phasing for solving the structure of biological macromolecules. The large value of the anomalous contribution of europium, f'' = 11.17 e(-), at the Cu Kα wavelength is an advantage in de novo phasing and automated model building. Tetragonal crystals of hen egg-white lysozyme (HEWL) incorporating europium(III) chloride (50 mM) were obtained which diffracted to a resolution of 2.3 Å at a wavelength of 1.54 Å (Cu Kα). The master data set (360° frames) was split and analyzed for anomalous signal-to-noise ratio, multiplicity, completeness, SAD phasing and automated building. The structure solution and model building of the split data sets were carried out using phenix.autosol and phenix.autobuild. The contributions of the Eu ions to SAD phasing using in-house data collection are discussed. This study revealed successful lysozyme phasing by SAD using laboratory-source data involving Eu ions, which are mainly coordinated by the side chains of Asn46, Asp52 and Asp101 together with some water molecules. PubMed: 23295480DOI: 10.1107/S1744309112047562 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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