4H1J

Crystal structure of PYK2 with the pyrazole 13a

Summary for 4H1J

• Entry DOI: 10.2210/pdb4h1j/pdb
• Related: 3FZS 4H1M
• Descriptor: Protein-tyrosine kinase 2-beta, 1-[3-tert-butyl-1-(4-methylphenyl)-1H-pyrazol-5-yl]-3-[3-(4-methoxy-2-methylphenyl)-1H-pyrazol-5-yl]urea (3 entities in total)
• Functional Keywords: kinase, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: Q14289
• Total number of polymer chains: 1
• Total formula weight: 34450.88

Authors

Han, S. (deposition date: 2012-09-10, release date: 2012-11-28, Last modification date: 2024-02-28)

Primary citation

Bhattacharya, S.K.,Aspnes, G.E.,Bagley, S.W.,Boehm, M.,Brosius, A.D.,Buckbinder, L.,Chang, J.S.,Dibrino, J.,Eng, H.,Frederick, K.S.,Griffith, D.A.,Griffor, M.C.,Guimaraes, C.R.,Guzman-Perez, A.,Han, S.,Kalgutkar, A.S.,Klug-McLeod, J.,Garcia-Irizarry, C.,Li, J.,Lippa, B.,Price, D.A.,Southers, J.A.,Walker, D.P.,Wei, L.,Xiao, J.,Zawistoski, M.P.,Zhao, X.
Identification of novel series of pyrazole and indole-urea based DFG-out PYK2 inhibitors.
Bioorg.Med.Chem.Lett., 22:7523-7529, 2012

PubMed Abstract: Previous drug discovery efforts identified classical PYK2 kinase inhibitors such as 2 and 3 that possess selectivity for PYK2 over its intra-family isoform FAK. Efforts to identify more kinome-selective chemical matter that stabilize a DFG-out conformation of the enzyme are described herein. Two sub-series of PYK2 inhibitors, an indole carboxamide-urea and a pyrazole-urea have been identified and found to have different binding interactions with the hinge region of PYK2. These leads proved to be more selective than the original classical inhibitors.

PubMed: 23153798
DOI: 10.1016/j.bmcl.2012.10.039

Experimental method

X-RAY DIFFRACTION (2 Å)