4GTB

T. Maritima FDTS with FAD, dUMP, and Raltitrexed.

Summary for 4GTB

• Entry DOI: 10.2210/pdb4gtb/pdb
• Descriptor: Thymidylate synthase thyX, FLAVIN-ADENINE DINUCLEOTIDE, 2'-DEOXYURIDINE 5'-MONOPHOSPHATE, ... (6 entities in total)
• Functional Keywords: flavin-dependent thymidylate synthase, tm0449, raltitrexed, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• Biological source: Thermotoga maritima
• Total number of polymer chains: 1
• Total formula weight: 29091.35

Authors

Mathews, I.I.,Lesley, S.A.,Kohen, A. (deposition date: 2012-08-28, release date: 2012-10-17, Last modification date: 2023-09-13)

Primary citation

Koehn, E.M.,Perissinotti, L.L.,Moghram, S.,Prabhakar, A.,Lesley, S.A.,Mathews, I.I.,Kohen, A.
Folate binding site of flavin-dependent thymidylate synthase.
Proc.Natl.Acad.Sci.USA, 109:15722-15727, 2012

PubMed Abstract: The DNA nucleotide thymidylate is synthesized by the enzyme thymidylate synthase, which catalyzes the reductive methylation of deoxyuridylate using the cofactor methylene-tetrahydrofolate (CH(2)H(4)folate). Most organisms, including humans, rely on the thyA- or TYMS-encoded classic thymidylate synthase, whereas, certain microorganisms, including all Rickettsia and other pathogens, use an alternative thyX-encoded flavin-dependent thymidylate synthase (FDTS). Although several crystal structures of FDTSs have been reported, the absence of a structure with folates limits understanding of the molecular mechanism and the scope of drug design for these enzymes. Here we present X-ray crystal structures of FDTS with several folate derivatives, which together with mutagenesis, kinetic analysis, and computer modeling shed light on the cofactor binding and function. The unique structural data will likely facilitate further elucidation of FDTSs' mechanism and the design of structure-based inhibitors as potential leads to new antimicrobial drugs.

PubMed: 23019356
DOI: 10.1073/pnas.1206077109

Experimental method

X-RAY DIFFRACTION (1.7 Å)