4GSX
High resolution structure of dengue virus serotype 1 sE containing stem
Summary for 4GSX
| Entry DOI | 10.2210/pdb4gsx/pdb |
| Related | 1OK8 1URZ 3G7T 4GT0 |
| Descriptor | Envelope protein E, 2-acetamido-2-deoxy-beta-D-glucopyranose, CADMIUM ION, ... (5 entities in total) |
| Functional Keywords | viral fusion protein, viral protein |
| Biological source | Dengue virus 1 (DENV-1) |
| Total number of polymer chains | 2 |
| Total formula weight | 94519.89 |
| Authors | Klein, D.E.,Choi, J.L.,Harrison, S.C. (deposition date: 2012-08-28, release date: 2012-12-19, Last modification date: 2024-10-16) |
| Primary citation | Klein, D.E.,Choi, J.L.,Harrison, S.C. Structure of a dengue virus envelope protein late-stage fusion intermediate. J.Virol., 87:2287-2293, 2013 Cited by PubMed Abstract: The final stages of dengue virus fusion are thought to occur when the membrane-proximal stem drives the transmembrane anchor of the viral envelope protein (E) toward the fusion loop, buried in the target cell membrane. Crystal structures of E have lacked this essential stem region. We expressed and crystallized soluble mutant forms of the dengue virus envelope protein (sE) that include portions of the juxtamembrane stem. Their structures represent late-stage fusion intermediates. The proximal part of the stem has both intra- and intermolecular interactions, so the chain "zips up" along the trimer seam. The penultimate interaction we detected involves the conserved residue F402, which has hydrophobic contacts with a conserved surface on domain II. These interactions do not require any larger-scale changes in trimer packing. The techniques for expression and crystallization of sE containing stem reported here may allow further characterization of the final stages of flavivirus fusion. PubMed: 23236058DOI: 10.1128/JVI.02957-12 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.903 Å) |
Structure validation
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