4GSX
High resolution structure of dengue virus serotype 1 sE containing stem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .979 |
| Spacegroup name | P 63 |
| Unit cell lengths | 77.571, 77.571, 292.518 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.130 - 1.903 |
| R-factor | 0.1658 |
| Rwork | 0.161 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g7t |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.104 | 0.445 |
| Number of reflections | 76924 | |
| <I/σ(I)> | 14.2 | 1.84 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 5.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
