4GGL

Pyrrolopyrimidine inhibitors of dna gyrase b and topoisomerase iv, part i: structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity

4GGL の概要

• エントリーDOI: 10.2210/pdb4ggl/pdb
• 関連するPDBエントリー: 4GEE 4GFN 4HXW 4HXZ 4HY1 4HYM 4HYP 4HZ0 4HZ5
• 分子名称: DNA gyrase subunit B, 7-({4-[(3R)-3-aminopyrrolidin-1-yl]-5-chloro-6-ethyl-7H-pyrrolo[2,3-d]pyrimidin-2-yl}sulfanyl)pyrido[2,3-b]pyrazin-2(1H)-one, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: atp-binding, nucleotide-binding, topoisomerase, atp-binding domain, dna gyrase negatively supercoils closed circular double-stranded dna in an atp-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded dna rings, isomerase-isomerase inhibitor complex, isomerase/isomerase inhibitor
• 由来する生物種: Enterococcus faecalis
• 細胞内の位置: Cytoplasm (By similarity): Q839Z1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24370.63

構造登録者

Bensen, D.C.,Creighton, C.J.,Tari, L.W. (登録日: 2012-08-06, 公開日: 2013-02-13, 最終更新日: 2024-02-28)

主引用文献

Tari, L.W.,Trzoss, M.,Bensen, D.C.,Li, X.,Chen, Z.,Lam, T.,Zhang, J.,Creighton, C.J.,Cunningham, M.L.,Kwan, B.,Stidham, M.,Shaw, K.J.,Lightstone, F.C.,Wong, S.E.,Nguyen, T.B.,Nix, J.,Finn, J.
Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity.
Bioorg.Med.Chem.Lett., 23:1529-1536, 2013

PubMed Abstract: The bacterial topoisomerases DNA gyrase (GyrB) and topoisomerase IV (ParE) are essential enzymes that control the topological state of DNA during replication. The high degree of conservation in the ATP-binding pockets of these enzymes make them appealing targets for broad-spectrum inhibitor development. A pyrrolopyrimidine scaffold was identified from a pharmacophore-based fragment screen with optimization potential. Structural characterization of inhibitor complexes conducted using selected GyrB/ParE orthologs aided in the identification of important steric, dynamic and compositional differences in the ATP-binding pockets of the targets, enabling the design of highly potent pyrrolopyrimidine inhibitors with broad enzymatic spectrum and dual targeting activity.

PubMed: 23352267
DOI: 10.1016/j.bmcl.2012.11.032

実験手法

X-RAY DIFFRACTION (1.69 Å)