4FLS

Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.

Summary for 4FLS

Entry DOI	10.2210/pdb4fls/pdb
Related	1G5A 4FLO 4FLQ 4FLR
Related PRD ID	PRD_900003
Descriptor	Amylosucrase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, CHLORIDE ION, ... (5 entities in total)
Functional Keywords	beta/alpha-barrel, glycoside hydrolase, amylose synthesis, sucrose isomerization, glucosyltransferase carbohydrate, transferase
Biological source	Neisseria polysaccharea
Cellular location	Secreted: Q9ZEU2
Total number of polymer chains	1
Total formula weight	72357.24
Authors	Guerin, F.,Champion, E.,Moulis, C.,Barbe, S.,Tran, T.H.,Morel, S.,Descroix, K.,Monsan, P.,Mulard, L.A.,Remaud-Simeon, M.,Andre, I.,Mourey, L.,Tranier, S. (deposition date: 2012-06-15, release date: 2012-10-31, Last modification date: 2023-09-13)
Primary citation	Champion, E.,Guerin, F.,Moulis, C.,Barbe, S.,Tran, T.H.,Morel, S.,Descroix, K.,Monsan, P.,Mourey, L.,Mulard, L.A.,Tranier, S.,Remaud-Simeon, M.,Andre, I. Applying pairwise combinations of amino Acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides. J.Am.Chem.Soc., 134:18677-18688, 2012 Cited by PubMed: 23072374 DOI: 10.1021/ja306845b PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.3 Å)

Structure validation