4FLS
Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Amylosucrase | polymer | 628 | 71545.1 | 1 | UniProt (Q9ZEU2) Pfam (PF00128) Pfam (PF22582) In PDB | Neisseria polysaccharea | |
| 2 | B, C | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900003) | sucrose | ||
| 3 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 4 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | water | water | 18.0 | 280 | Chemie (HOH) |
Sequence modifications
A: 5 - 628 (UniProt: Q9ZEU2)
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Asn 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Lys 290 | Phe 298 | engineered mutation |
| Gln 328 | Glu 336 | engineered mutation |
| Asp 537 | Gly 545 | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 71545.1 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 127.5 | |
| All* | Total formula weight | 72357.2 |
