4FEW

Crystal structure of the aminoglycoside phosphotransferase APH(3')-Ia, with substrate kanamycin and small molecule inhibitor pyrazolopyrimidine PP2

Summary for 4FEW

• Entry DOI: 10.2210/pdb4few/pdb
• Related: 4EJ7 4GKH 4GKI 4feu 4fev 4fex
• Descriptor: Aminoglycoside 3'-phosphotransferase AphA1-IAB, KANAMYCIN A, 1-TERT-BUTYL-3-(4-CHLORO-PHENYL)-1H-PYRAZOLO[3,4-D]PYRIMIDIN-4-YLAMINE, ... (8 entities in total)
• Functional Keywords: pyrazolopyrimidine, pp2, protein kinase inhibitor, center for structural genomics of infectious diseases, csgid, niaid, national institute of allergy and infectious diseases, eukaryotic protein kinase-like fold, alpha/beta protein, transferase, phosphotransferase, aminoglycoside phosphotransferase, antibiotic resistance, aminoglycosides, kanamycin, gtp, intracellular, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• Biological source: Acinetobacter baumannii
• Total number of polymer chains: 6
• Total formula weight: 193589.00

Authors

Stogios, P.J.,Evdokimova, E.,Wawrzak, Z.,Minasov, G.,Egorova, O.,Di Leo, R.,Shakya, T.,Spanogiannopoulos, P.,Wright, G.D.,Savchenko, A.,Anderson, W.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (deposition date: 2012-05-30, release date: 2012-06-20, Last modification date: 2023-12-06)

• Primary citation: Stogios, P.J.,Spanogiannopoulos, P.,Evdokimova, E.,Egorova, O.,Shakya, T.,Todorovic, N.,Capretta, A.,Wright, G.D.,Savchenko, A.; Structure-guided optimization of protein kinase inhibitors reverses aminoglycoside antibiotic resistance.; Biochem.J., 454:191-200, 2013; PubMed: 23758273; DOI: 10.1042/BJ20130317

Experimental method

X-RAY DIFFRACTION (1.98 Å)