4FEG

High-resolution structure of pyruvate oxidase in complex with reaction intermediate 2-hydroxyethyl-thiamin diphosphate carbanion-enamine, crystal A

Summary for 4FEG

Entry DOI	10.2210/pdb4feg/pdb
Related	1POW 1POX 2EZ4 2EZ8 2EZ9 2EZT 2EZU 4FEE
Descriptor	Pyruvate oxidase, PYRUVIC ACID, PHOSPHATE ION, ... (8 entities in total)
Functional Keywords	carbanion, structure activity relationship, oxidation-reduction, umpolung, thiamine diphosphate, reaction intermediate, oxidoreductase
Biological source	Lactobacillus plantarum
Total number of polymer chains	2
Total formula weight	136248.96
Authors	Meyer, D.,Neumann, P.,Koers, E.,Sjuts, H.,Luedtke, S.,Sheldrick, G.M.,Ficner, R.,Tittmann, K. (deposition date: 2012-05-30, release date: 2012-06-20, Last modification date: 2023-11-15)
Primary citation	Meyer, D.,Neumann, P.,Koers, E.,Sjuts, H.,Ludtke, S.,Sheldrick, G.M.,Ficner, R.,Tittmann, K. Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin. Proc.Natl.Acad.Sci.USA, 109:10867-10872, 2012 Cited by PubMed: 22730460 DOI: 10.1073/pnas.1201280109 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.09 Å)

Structure validation