4F7E

Crystal structure of bovine CD1d with bound C16:0-alpha-galactosyl ceramide

Summary for 4F7E

• Entry DOI: 10.2210/pdb4f7e/pdb
• Related: 1Z5L 1ZT4 4F7C
• Descriptor: CD1D antigen, d polypeptide, Beta-2-microglobulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: protein-glycolipid complex, mhc-fold, ig-fold, antigen presentation, tcr, membrane, immune system
• Biological source: Bos taurus (bovine); More
• Cellular location: Secreted: P01888
• Total number of polymer chains: 2
• Total formula weight: 45573.44

Authors

Wang, J.,Zajonc, D.M. (deposition date: 2012-05-15, release date: 2012-11-14, Last modification date: 2024-10-30)

Primary citation

Wang, J.,Guillaume, J.,Pauwels, N.,Van Calenbergh, S.,Van Rhijn, I.,Zajonc, D.M.
Crystal Structures of Bovine CD1d Reveal Altered αGalCer Presentation and a Restricted A' Pocket Unable to Bind Long-Chain Glycolipids.
Plos One, 7:e47989-e47989, 2012

PubMed Abstract: NKT cells play important roles in immune surveillance. They rapidly respond to pathogens by detecting microbial glycolipids when presented by the non-classical MHC I homolog CD1d. Previously, ruminants were considered to lack NKT cells due to the lack of a functional CD1D gene. However, recent data suggest that cattle express CD1d with unknown function. In an attempt to characterize the function of bovine CD1d, we assessed the lipid binding properties of recombinant Bos taurus CD1d (boCD1d) in vitro. BoCD1d is able to bind glycosphingolipids (GSLs) with fatty acid chain lengths of C₁₈, while GSLs with fatty acids of C₂₄ do not bind. Crystal structures of boCD1d bound to a short-chain C₁₂-di-sulfatide antigen, as well as short-chain C₁₆-αGalCer revealed that the Á pocket of boCD1d is restricted in size compared to that of both mouse and human CD1d, explaining the inability of long chain GSL's to bind to boCD1d. Moreover, while di-sulfatide is presented similarly compared to the presentation of sulfatide by mouse CD1d, αGalCer is presented differently at the cell surface, due to an amino acid Asp151Asn substitution that results in loss of intimate contacts between the αGalCer headgroup and CD1d. The altered αGalCer presentation by boCD1d also explains its lack of cross-activation of mouse iNKT cells and raises the interesting question of the nature and function of bovine lipid-reactive T cells.

PubMed: 23110152
DOI: 10.1371/journal.pone.0047989

Experimental method

X-RAY DIFFRACTION (2.4 Å)