4F7E
Crystal structure of bovine CD1d with bound C16:0-alpha-galactosyl ceramide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.785, 74.479, 122.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.900 - 2.400 |
R-factor | 0.2233 |
Rwork | 0.221 |
R-free | 0.27630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l9r |
RMSD bond length | 0.011 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.067 | 0.199 |
Number of reflections | 20240 | |
<I/σ(I)> | 35 | 4.9 |
Completeness [%] | 97.4 | 98.2 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% PEG4000, 200 mM potassium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |