4F02

Crystal structure of the PABP-binding site of eIF4G in complex with RRM1-2 of PABP and poly(A)

4F02 の概要

• エントリーDOI: 10.2210/pdb4f02/pdb
• 関連するPDBエントリー: 4F25 4F26
• 分子名称: Polyadenylate-binding protein 1, RNA (5'-R(*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3'), Eukaryotic translation initiation factor 4 gamma 1, ... (4 entities in total)
• 機能のキーワード: translation initiation, mrna, eukaryotic initiation factors (eifs), paip1 and paip2, translation-rna complex, translation/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm : P11940
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 60871.69

構造登録者

Safaee, N.,Kozlov, G.,Gehring, K.B. (登録日: 2012-05-03, 公開日: 2012-08-08, 最終更新日: 2024-02-28)

主引用文献

Safaee, N.,Kozlov, G.,Noronha, A.M.,Xie, J.,Wilds, C.J.,Gehring, K.
Interdomain Allostery Promotes Assembly of the Poly(A) mRNA Complex with PABP and eIF4G.
Mol.Cell, 48:375-386, 2012

PubMed Abstract: Many RNA-binding proteins contain multiple single-strand nucleic acid-binding domains and assemble into large multiprotein messenger ribonucleic acid protein (mRNP) complexes. The mechanisms underlying the self-assembly of these complexes are largely unknown. In eukaryotes, the association of the translation factors polyadenylate-binding protein-1 (PABP) and eIF4G is essential for high-level expression of polyadenylated mRNAs. Here, we report the crystal structure of the ternary complex poly(A)(11)·PABP(1-190)·eIF4G(178-203) at 2.0 Å resolution. Our NMR and crystallographic data show that eIF4G interacts with the RRM2 domain of PABP. Analysis of the interaction by small-angle X-ray scattering, isothermal titration calorimetry, and electromobility shift assays reveals that this interaction is allosterically regulated by poly(A) binding to PABP. Furthermore, we have confirmed the importance of poly(A) for the endogenous PABP and eIF4G interaction in immunoprecipitation experiments using HeLa cell extracts. Our findings reveal interdomain allostery as a mechanism for cooperative assembly of RNP complexes.

PubMed: 23041282
DOI: 10.1016/j.molcel.2012.09.001

実験手法

X-RAY DIFFRACTION (2 Å)